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Microorganisms able to live in oxygenated environments produce enzymes which neutralize toxic forms of oxygen. One such enzyme is catalase, which breaks hydrogen peroxide into water and molecular oxygen. Organisms which produce catalase will bubble when placed into hydrogen peroxide. Enzymes contain a cavity called the active site, to which the substrate, the substance whose reaction is catalyzed by the enzyme, bonds, forming the enzyme-substrate complex. Only one type of substrate can bond with the enzyme, in a lock-and-key manner. The enzyme then breaks the substrate down to form the products of the organic reaction. Enzymes are effected by temperature, ph, and substrate to enzyme concentration. adding heat causes reactions to occur faster. however, adding too much heat will cause the enzymes to denature and lose their function. Purpose: The purpose of this experiment is to determine the effect of temperature on enzyme function.
The enzyme-linked immunosorbent assay (ELISA) is a laboratory technique that detects and measures specific proteins. It can show whether a patient has antibodies related to certain diseases, making it useful for medical diagnoses. In this lab, students perform a virtual ELISA to test whether a particular antibody is present in a blood sample. In the Enzyme Kinetics Lab, you will learn how substrates are converted into products by catalysis. You will also learn all about the kinetics of enzyme involving the Michaelis-Menten equation and various rate constants, as well as DNA mutation and hyperactivity.
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During this bacterial transformation lab, we inserted a plasmid containing a antibiotic-resistance gene. The goal of the lab was to identify the plasmids because it was unknown which plasmids contained which genes. Each group had a different plasmid to test. We tested for ampicillin resistance, kanamycin resistance, and the presence of the GFP.
Enzyme Activity of Salivary Amylase Page 5 Name: _____ Pre‐lab Questions: Enzyme Activity of Salivary Amylase 1. What is the relationship between starch, dextrins and glucose? 2. What is the job of an enzyme? 3. Draw an energy diagram that shows the energy in a chemical reaction with and without an enzyme. 4. Original Document: Enzyme Lab. MATERIALS: The chemicals can be purchased from chemical or biological supply companies, students should wear safety goggles This lab requires some set up and can be messy. Not advised unless you actually have a lab to work within. There is an alternative lab that uses a website at enzymelabweb.html In the virtual lab window to the left, select experiment 1 to begin this portion of the virtual lab. You must complete all three experiments in order along with the accompanying questions below. 2.
You can modify this lab to test the effect of enzyme concentration, pH, or salinity. Similarly, you can follow up this experiment by having students designing their own experiment to test one of these factors. Enzyme Activity: With 3ml H 2 O 2 and 3ml H 2 O in each tube, add 1 drop of enzyme suspension. Repeat with 2, 3, and 4 drops. Enzyme kinetics of Turnip Peroxidase. Hydrogen peroxide (H 2 O 2) is a strong oxidizing agent that can damage cells and is formed as a by-product of oxygen consumption. Fortunately, aerobic cells contain peroxidases that break down peroxide into water and oxygen. This enzyme reduces hydrogen peroxide into H 2 O by oxidizing an organic compound ... biogeochemical lab. CLIMATOGRAM. Eating at a trophic level. Powered by Create your own unique website with customizable templates. Get Started. Living Environment Apr 20, 2017 · AP BIOLOGY WEBLABS – This site has a virtual lab on each of the “dirty dozen” AP Biology labs. A great time saver ! MCGRAW-HILL VIRTUAL LABS – The McGraw Hill virtual lab is great — just print off the post lab questions and tables and have students complete the lab. AP ENVIRONMENTAL … Continue reading "Virtual Labs"
Photosynthesis virtual lab answer key quizlet. Photosynthesis virtual lab answer key quizlet ... PHYSIOLOGY AND MAINTENANCE – Vol. II – Enzymes: The Biological Catalysts of Life - Pekka Mäntsälä and Jarmo Niemi ©Encyclopedia of Life Support Systems (EOLSS) Generally, an increase in temperature increases the activity of enzymes. Because enzymes function in cells, the optimum conditions for most enzymes are moderate temperatures.